Molecular biology and cancer researches


A professional lecturer and Researcher with expertise in traditional and modern research methodologies. Skilled in providing strategic solutions and assessing project performance. Hands-on experience completing development research and leading detailed competitive assessments.
Dedicated researcher familiar with biotechnology protocols. Well-coordinated in handling study documentation and records. Brings more than 6 years of academic and research experience.
1 . Cytotoxicity cell culture for testing synthetic materials prior to implementation in experimental animals
2. Western blot testing
3. qPCR testing
4. Cells migration and invasion assays
5. Histology and cytology testing
6. Immunohistochemistry testing
7. Immunoflouresence testing
8. Drugs cytotoxicity and inhibitory effects testing
DNA and RNA extraction
cDNA synthesis
Cell culture in monolayer – 2D cultures
Cell culture in Spheroids – 3D culture
Cell culture in organoids – 3D culture
Cell culture on scaffolds – Synthetic materials
Primary cell culture from tumors and normal tissues – human and animals
DNA and RNA gel electrophoresis
Quantitative real time PCR (qPCR)
Western Blot
ELISA
Immuno-florescence assays
Immunohistochemistry assays
MTT and Presto-blue viability testing
Cells cytotoxicity testing against drugs, synthetic materials, plant extracts, and metabolites
Migration assay for cancer cells and normal cells
Clinical trials on animals, including Mice, Rats, and Rabbits
LCMS-MS
Molecular biology and cancer researches