Summary
Overview
Work History
Education
Skills
Additional Information
Certification
Interests
Timeline
Training Courses
Diving certification and work experience
Others
Notes
Publications
Generic
Anan Ishtiah

Anan Ishtiah

Lecturer And Researcher
Amman

Summary

A professional lecturer and Researcher with expertise in traditional and modern research methodologies. Skilled in providing strategic solutions and assessing project performance. Hands-on experience completing development research and leading detailed competitive assessments.

Dedicated researcher familiar with biotechnology protocols. Well-coordinated in handling study documentation and records. Brings more than 6 years of academic and research experience.

Overview

20
20
years of professional experience
1
1
Certification
3
3
Languages

Work History

Research Analyst

Advanced Medical and Dental Institute (AMDI), University Sains Malaysis (USM)
10.2021 - 12.2024
  • The leader of Dr. Badrul Hisham bin Yahaya service program that providing samples testing for researchers and research facilities inside USM, and outside USM.
  • Conducting experiments in different fields of biotechnology including:

1 . Cytotoxicity cell culture for testing synthetic materials prior to implementation in experimental animals

2. Western blot testing

3. qPCR testing

4. Cells migration and invasion assays

5. Histology and cytology testing

6. Immunohistochemistry testing

7. Immunoflouresence testing

8. Drugs cytotoxicity and inhibitory effects testing

Research Assistant

Animal Research Complex, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM)
10.2020 - 02.2024
  • Conducting experiments on Mice, Rats, and Rabbets
  • Animals daily care
  • Operation: including Trachea transplant, and Mesenchymal stem cells implanting
  • Schaffolds transplantation
  • Giving drugs as injections
  • Euthanasia, Blood and organs collections
  • Toxicity testing
  • Histology testing
  • Primary cell cultures
  • Organoids cultures

Academic: Lecturer

University of Tabuk
10.2011 - 06.2018
  • Teaching, Theory and Practical, mainly:
  • Molecular Biology
  • Human Genetics
  • Hematology
  • Blood Bank and Transfusion Medicine
  • Member of the (Community Service & Student Affairs Committee)
  • Member of Accreditation committee
  • Member of the (Hospitals Clinical Rotation & Internship Committee)
  • Three years supervising students at clinical training in hospitals

Research Assistant

Prince Fahad bin Sultan Research chair
10.2011 - 06.2018
  • Research involvement (2016):
  • Genetic alterations in the pathogenesis of myeloproliferative disorders among MPD patients of Tabuk-Saudi Arabia ( Screening MPD patients for mutations in the following genes → Jak2 V617F, Jak2 exon 12, CALR, and MPL)
  • Genetic alterations in the pathogenesis of Breast cancer patients of Tabuk region-Saudi Arabia (hTERT and BRCA1/2 genes) – Hotspot mutations, SNPs association, and Promoter methylation.

Diagnostic

Prince Fahad Bin Sultan Research Center
10.2011 - 06.2018
  • Member of the Molecular Diagnostic unit at Prince Fahad Bin Sultan Research Center, & Performing the following Tests:
  • Leukemia: JAK2 mutations, BCR-ABL gene, (T315I) mutation, FLT3-ITD, FLT3 RTK-D835, NPM mutation, CALR 1367fs 46 deletion, CALR K385fs 47 insertion
  • Chromosomal Translocations : t(9;22)(q21;q11) BCR-ABL fusion, t(15;17)(q21;q22) AML1-ETO fusion, t(8;14)(q24;q32) MYC
  • Lung Cancer Panel : EGFR Exon 19 deletion, EGFR Exon 20 mutation, EGFR Exon 21, AKT1 c.49G
  • Colon Cancer Panel : KRAS
  • Breast Cancer Panel : PIK3CA mutation, PIK3CA E542K, E545K exon 9, BRCA1/2.

Medical & Scientific Officer

BabyCord Jordan
07.2009 - 08.2011
  • Cord Blood collection
  • Cord blood collection
  • Cells counting
  • Nurses training – Including :
  • Cord blood standardized collection methods
  • Sterilization, safety & quality control
  • Responsible for the Standard Operational Procedure (SOP) fulfillment, validation and development for the Nurses, the Medical Representatives, and all the work done by the center.
  • New Collection techniques & methods development.
  • Training & experimenting new collection methods at Al-Amal Maternity hospital, these methods adds up to 30% for the cells count collected by the traditional methods.
  • Lectures Presentation
  • I have represented BabyCord Jordan in many conference including:
  • Umbilical cord blood banking – Stem Cells Conference Held at the Hashimite University. 2009, Zarka, Jordan
  • Cord blood : the hidden treasure of life – Stem Cells conference held at Amman Arab University – 3/2009, Amman, Jordan
  • Stem cells : Applications in oral and maxillofacial surgery – Oro - maxillofacial surgery society conference – 2010, Amman, Jordan
  • Cord Blood Banking – for Jordan Food society: Public seminar2009
  • Many other lectures for gynecologists, mothers, & pregnant.
  • FDA, FACTS, & AABB Regulations: studying, translation, and work through them as a guidance in all our work
  • An affiliate of New England Cord Blood Bank (NECBB) – Boston - USA

Medical Technologist

King Abdulla University Hospital – Jordan University of Science and Technology
12.2007 - 07.2010
  • Daily preparation of laboratory analyzers, calibration and maintenance.
  • Daily run and monitoring of internal laboratory controls.
  • Analyzing patient’s samples for almost all hematological, body fluids, and coagulation tests.
  • Evaluating of external laboratory controls.
  • Working on reduction of laboratory error, for example diminishing of carryover problem particularly in pediatric samples.
  • The auto-analyzer that are available in our department and I have a good experience on working with: Sysmex, Pentra, Stago.
  • The Hospital is accredited to JCIA QA program: I have a training course on JCIA standards.
  • Jordan, JCIA accredited

Medical Technologist

Al-Amal Maternity Hospital – MedLabs, Jordan
07.2008 - 08.2009
  • Daily preparation of laboratory all analyzers, calibration and maintenance.
  • Daily run and monitoring of internal laboratory control.
  • Analyzing of patients samples for almost all chemical, hormonal, hematological, Fluids, and microbiological tests.
  • Evaluating of external laboratory control (from Medlabs, Biorad).
  • Blood collection from all age groups including neonates.

Geneticist

Al-Amal Maternity Hospital – MedLabs, Jordan
07.2006 - 11.2007
  • Clinical diagnosis of many diseases and mutations such as MTHFR, Factor V Leiden, Hemophelia, Thalasemia, and others
  • Nucleic acid extraction, amplification and detection
  • Cell culture & Cytogenetic analysis
  • FISH technique : Training only
  • PGD training workshop
  • Training on Genetic counseling

Medical Technologist

Al-Ahmed Medical Laboratory, Jordan
10.2005 - 07.2006
  • Daily preparation of laboratory all analyzers, calibration and maintenance.
  • Daily run and monitoring of internal laboratory control.
  • Analyzing of patients samples for almost all chemical, hormonal, hematological, Fluids, and microbiological tests.

Medical Technologist

MedLabs – Aljuda Main Laboratory, Jordan
06.2005 - 10.2005
  • Daily preparation of laboratory all analyzers, calibration and maintenance.
  • Daily run and monitoring of internal laboratory control.
  • Analyzing of patients samples for almost all chemical, hormonal, hematological, Fluids, and microbiological tests.
  • Dealing with referral samples from other laboratories all over the country.

Education

PhD - Medical Genetics – specialty in Cancer Genetics

University Sains Malaysia (USM)
Penang, Malaysia
01-2026

Master degree - Medical Technology - Diagnostic Molecular Biology and Human Genetics

Jordan University of Science and Technology (JUST)
Irbid, Jordan
01-2011

Bachelor’s degree - Medical Laboratory Technology

Jordan University of Science and Technology (JUST)
Irbid, Jordan
01-2005

Skills

DNA and RNA extraction

cDNA synthesis

Cell culture in monolayer – 2D cultures

Cell culture in Spheroids – 3D culture

Cell culture in organoids – 3D culture

Cell culture on scaffolds – Synthetic materials

Primary cell culture from tumors and normal tissues – human and animals

DNA and RNA gel electrophoresis

Quantitative real time PCR (qPCR)

Western Blot

ELISA

Immuno-florescence assays

Immunohistochemistry assays

MTT and Presto-blue viability testing

Cells cytotoxicity testing against drugs, synthetic materials, plant extracts, and metabolites

Migration assay for cancer cells and normal cells

Clinical trials on animals, including Mice, Rats, and Rabbits

LCMS-MS

Additional Information

  • Hands-on training at animal research complex for experimental animals care at Advanced medical and dental institute, Universiti sains malaysis. (October 2019).
  • "Hands-on workshop on real-time cell analysis: for analysis of cultured cells in real time conducted at animal research complex at Advanced medical and dental institute, Universiti sains malaysis. (October 2018)
  • “Second Generation sequencing” - Center of Excellence In Genomic Medicine Research – King Abdulaziz University, Jeddah, Saudi Arabia (26/11/2014)
  • Comprehensive training program as part of training courses in the fourth year of bachelor's degree for one-year that includes departments of (Microbiology, Parasitology and body fluid analysis laboratory, biochemistry and endocrinology lab, serology lab, hematology lab & blood bank).
  • “PCR Technology” Principles and Diagnostic Applications. University of Jordan, Amman-Jordan. ( 23/02/2006 )
  • “Testing and Evaluation: Towards Improving Instructions” , University of Tabuk, Saudi Arabia (20/02/2012)
  • Four months training course at King Abdulla University Hospital at the Cytogenetics Departments including: Cell culture, Cell harvesting, and Karyotyping (2011)

Certification

I have a certificate of Medical laboratory specialist from the ministry of health in Jordan ( مزاولة مهنة – اختصاصي مختبرات طبية )

Interests

Molecular biology and cancer researches

Timeline

Research Analyst

Advanced Medical and Dental Institute (AMDI), University Sains Malaysis (USM)
10.2021 - 12.2024

Research Assistant

Animal Research Complex, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM)
10.2020 - 02.2024

Academic: Lecturer

University of Tabuk
10.2011 - 06.2018

Research Assistant

Prince Fahad bin Sultan Research chair
10.2011 - 06.2018

Diagnostic

Prince Fahad Bin Sultan Research Center
10.2011 - 06.2018

Medical & Scientific Officer

BabyCord Jordan
07.2009 - 08.2011

Medical Technologist

Al-Amal Maternity Hospital – MedLabs, Jordan
07.2008 - 08.2009

Medical Technologist

King Abdulla University Hospital – Jordan University of Science and Technology
12.2007 - 07.2010

Geneticist

Al-Amal Maternity Hospital – MedLabs, Jordan
07.2006 - 11.2007

Medical Technologist

Al-Ahmed Medical Laboratory, Jordan
10.2005 - 07.2006

Medical Technologist

MedLabs – Aljuda Main Laboratory, Jordan
06.2005 - 10.2005

Master degree - Medical Technology - Diagnostic Molecular Biology and Human Genetics

Jordan University of Science and Technology (JUST)

Bachelor’s degree - Medical Laboratory Technology

Jordan University of Science and Technology (JUST)

PhD - Medical Genetics – specialty in Cancer Genetics

University Sains Malaysia (USM)

Training Courses

  • Second Generation sequencing - Center of Excellence In Genomic Medicine Research – King Abdulaziz University, Jeddah, Saudi Arabia (26/11/2014)
  • Comprehensive training program at (as part of training courses in the fourth year of bachelor’s) – one-year training that includes departments of (Microbiology, Parasitology and body fluid analysis laboratory, biochemistry and endocrinology lab, serology lab, hematology lab & blood bank.
  • PCR Technology” Principles and Diagnostic Applications. University of Jordan, Amman-Jordan. ( 23/02/2006 )
  • Testing and Evaluation: Towards Improving Instructions” , University of Tabuk, Saudi Arabia (20/02/2012)
  • Four months training course at King Abdulla University Hospital at the Cytogenetics Departments including the following
  • Cell culture
  • Cell harvesting
  • Karyotyping slides preparation and staining
  • Karyotypeing analysis of metaphase chromosomes for genetic disorders.
  • JCIA Quality Assurance training program at King Abdulla University Hospital during the time of employment.
  • NCAAA Accreditation Program at University of Tabuk During time of Employment.
  • Training program & workshops in the university of tabuk, for the accreditation by the National Commission for Academic Accreditation & Assessment (NCAAA), 16/12/2012, including
  • Enhancing the competence of teachers in the health profession
  • Bulding up toward accreditation

Diving certification and work experience

  • Certificate: PADI Open Water Scuba Instructor / Instructor Number: 412795 Emergency First Response Instructor - EFR
  • Date: 04/2018
  • Status : Active Teaching Status
  • Institution: PADI Europe and Middle East
  • Work experience :
  • Scuba Diving instructor at Dive Time – Scuba Diving Center (Tabuk – Saudi Arabia)
  • EFR at Dive Time – Scuba Diving Center (Tabuk – Saudi Arabia)
  • Levels of training:
  • Open water Diving: training and licensing of open water divers, advanced open water divers, adventure divers, and rescue divers)
  • Specialty Diving: I have trained and licensed specialty divers, including Nitrox Diving, Night Diving, Search and Recovery Diving, Peak Performance Buoyancy Diving, and deep diving
  • EFR courses: I have held and licensed 4 EFR classes.
  • I have more than 400 dives in different water environments and times, including Deep dives (140 feet), dives in currents, search and recovery dives, caves dives, wreck dives, and night dives

Others

  • I have a good computer skill and a car license.
  • I have a very good English language and I have passed the IELTS Exam at British council – Amman – Jordan with a score of 6.5
  • I hold PADI Open Water Scuba diving license, from the Royal Diving Club Jordan, 2012
  • I hold PADI Advanced Open Water Diving License, Tabuk, Saudi Arabia, (11/11/2016)

Notes

Some of my working experiences in Jordan are overlapping because most of the time I was working in double works, one of them as full time and the other as part time.

Publications

  • I S Mahmoud, M A Sughayer, H A Mohammad, A A Eshtayeh, A S Awidi, M S EL-Khateeb & S I Ismail. The transforming mutation E17K/AKT1 is not a major event in B-cell-derived lymphoid leukaemias. Br J Cancer 99, 488–490 (2008). https://doi.org/10.1038/sj.bjc.6604512
  • Ishtiah, A.A., Yahaya, B.H. (2022). The Enrichment of Breast Cancer Stem Cells from MCF7 Breast Cancer Cell Line Using Spheroid Culture Technique. In: Kannan, N., Beer, P. (eds) Stem Cell Assays. Methods in Molecular Biology, vol 2429. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1979-7_32
  • Shaffi, S.C., Zakaria, N., Halim, N.S.S.A., Ishtiah, A.A., Patar, A.A., Yahaya, B.H. (2023). The Rapid Development of Airway Organoids: A Direct Culture Strategy. In: Pham, P.V. (eds) Advances in Mesenchymal Stem Cells and Tissue Engineering. ICRRM 2023. Advances in Experimental Medicine and Biology(). Springer, Cham. https://doi.org/10.1007/5584_2023_767
  • Anan A. Ishtiah, Omar Nafis Hairuddin, Badrul Hisham Yahaya. Unlocking the Potential of 3D Spheroid Cultures in Breast Cancer Stem Cell Enrichment and Isolation. Eurasian Journal of Medicine and Oncology 2024, 8(3), 322–335. https://doi.org/10.14744/ejmo.2024.97120
  • Yahaya BH, Bakar NFAA, Samat AA, Ishtiah AA, Yusof LM, Hamid ZAA, Osman ND, Khalil NA, Radzi RM, Musa MY, Jaafar M. Collagen-Coated 3D-TechTra Scaffold Integrated with Human Umbilical Cord Mesenchymal Stem Cells Enhances Tracheal Tissue Regeneration and Reduces Stenosis in Rabbit Models. Curr Med Sci. 2022 Oct;45(5):1123-1137. doi: 10.1007/s11596-025-00119-y. Epub 2025 Oct 8. PMID: 41060357.
Anan IshtiahLecturer And Researcher